|
|
| |
|
|
|
| |
|
| |
|
Phylonix is a Contract Research Organization providing
in vivo zebrafish assays for drug discovery and screening. Our toxicologists,
biochemists, developmental biologists, aquaculturists and molecular
biologists can perform general, organ and cell specific toxicity studies,
pathology, gene profiling, cellular activity and behavioral studies.
Custom assays can be designed for high throughput quantitative microplate
analysis or multi-parameter visual assessment. |
|
| |
|
|
|
| |
|
A New Era In Animal
Testing |
|
| |
|
Zebrafish develop rapidly. Three days after
fertilization, the embryo is essentially complete, with a functioning
heart, circulatory and nervous system. This rapid development is comparable
to three months of human development. In addition, zebrafish have
a relatively short generation time (2-3 months) and produce large
clutches of embryos (100-200) per mating.
By day four, the zebrafish embryo has hatched
and can eat and swim. Intestinal epithelial cells are polarized
and express digestive enzymes. Hepatocytes secrete bile. Pancreatic
islets and acini produce insulin and carboxypeptidase. Since zebrafish
embryos are transparent, every event in early development can be
observed visually. Quantitative endpoint assays can be performed
using microplate readers, similar to cell based formats.
|
|
| |
|
|
|
| |
|
Aquacultural Facilities |
|
| |
|
Phylonix is operating a state of the art aquaculture
facility with the capacity to produce thousands of embryos per week.
|
|
| |
|
|
|
| |
|
Services Include |
|
| |
|
- General Toxicity
- Specific Toxicity
- Cellular Activity
- Genetic Profiling
- Genetic Toxicology
- Pathology
- Behavioral Studies
- Custom Designed Experiments
|
|
| |
|
|
|
| |
|
Advantage of Drug
Testing in Zebrafish |
|
| |
|
Zebrafish currently rival mice and rats as a popular
laboratory animal model. Interest in zebrafish for drug screening
is rapidly increasing due to several inherent advantages, including:
- Short Experimental Time
- Access to all Developmental Stages
- Easy Manipulation for Automated or Visual Screens
- Drug Administration Directly to Fish Water
- Or by Microinjection
- Rapid Vertebrate Organogenesis
- Statistically Significant Number of Animals
per Test
- Small Amount of Drug Required
- Low Cost
|
|
| |
|
|
|
|
