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We offer quantitative microplate based whole animal High Throughput Screening Services for evaluating drug effects on Angiogenesis, Apoptosis and Toxicity. We have combined liquid sample handling devices and microplate readers to analyze large chemical libraries. Small molecules are delivered directly in the fish water and proteins are injected. Additional effects of “hits” can be assessed visually. |
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Advantages of Zebrafish for HTS |
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| Delivery of small molecules by direct addition
to the embryo in a microwell is an advantage for use with automated
sample handling instrumentation. The ability to screen large libraries
and rapidly assess statistically significant numbers of animals are
important advantages of our approach. High throughput screening of
whole animals after drug treatment will complement testing in cell
culture and mice. Assessment of drug effects in a convenient vertebrate
model, prior to proceeding to evaluation in mice, will streamline
drug development and dramatically reduce costs. |
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Advantages Include: |
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Angiogenesis |
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| We use a rapid, enzyme specific primary drug screen and a secondary vessel specific antibody based ELISA screen. We analyze whole embryos in a quantitative 96-microwell format or in a multiparameter visual screen. An in vivo microangiography assay is used for validation and our array analysis can detect vessel-specific gene expression. | |||
Apoptosis |
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We use fluorescent staining to assess the sites of apoptosis in vivo. The cell type and the degree of apoptosis is assessed by flow cytometry or fluorescence microplate assays. We can precisely detect apoptotic activity in whole embryos and rank potential apoptotic activators and inhibitors. Additional High Throughput Screens, Including Toxicity, Are Available. Please Inquire.
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